PathHunter® eXpress CCR2 CHO-K1 β-Arrestin GPCR Assay

Cells were plated in a 96-well plate and stimulated with a control agonist. Following stimulation, signal was detected according to the recommended protocol.

The PathHunter® eXpress CCR2 CHO-K1 β-Arrestin GPCR Assay measures CCR2 (GPCR) activity via recruitment of β-Arrestin 2. eXpress kits contain all assay materials: cells, reagents, and plates.

Catalog #: 93-0192E2CP0L

$2,279.00 – $7,479.00

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Biological Information
Background Information:
The PathHunter® CCR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL2/MCP-1) based activation of CCR2 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The cells included overexpress PK-tagged CCR2 and EA-tagged β-Arrestin-2. Activation of CCR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
GPCR
Family:
Chemokine Receptors
Sub Family:
Class A
Coupling:
Gi/Go
Accession Number:
NM_001123396
Target Name:
CCR2 (MIP-1R)
Target Aliases:
Chemokine (C-C motif) receptor 2CC-CKR-2CCR-2CCR2ACCR2BCD192CKR2CKR2ACKR2BCMKBR2MCP-1-R
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
eXpress Assay Kits
Application:
Drug Discovery & Development
Storage Conditions:
Short term (24 h): Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
β-Arrestin isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-255 PathHunter B Arrestin eXpress GPCR Assay REV4 User Manual

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Datasheets

93-0192E2_PathHunter_eXpress CCR2 CHO-K1 beta-Arrestin GPCR Assay

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