PathHunter® eXpress c-Ret-GFRα2 Functional Assay

Background Information:

The PathHunter® eXpress c-Ret-GFRα2 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated c-Ret and GFRα2 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The cells included overexpress PK-tagged c-Ret and an EA-tagged SH2 domain protein. It also overexpresses an untagged co-receptor, GFRα2 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Cyno version of the protein.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

Ret

Protein Aliases:

cadherin-related family member 16|RET receptor tyrosine kinase|rearranged during transfection

Accession Number:

NM_020975.4

UniProt Number:

P07949

Gene Name:

RET

Gene ID:

5979

Gene Aliases:

PTC|CDHF12|RET51|CDHR16

Target Species:

Human

Cell Background:

U2OS