PathHunter® eXpress BLK Activity Assay

The PathHunter® eXpress BLK Activity Assay measures BLK CTK activity via ligand-induced SH2 domain protein recruitment. eXpress kits contain all assay materials: cells, reagents, and plates.

Catalog #: 93-0882E3CP16L


Biological Information
Background Information:
The PathHunter® eXpress BLK Activity Assay provides an easy-to-use cell based assay to study small molecule drug candidates targeting CTKs. This kit assesses CTK activation via detection of resulting GHR phosphorylation-induced SH2 domain protein recruitment, mediated by BLK-JAK2 fusion protein. The cells included overexpress PK-tagged relevant cytokine receptor and EA-tagged SH2 domain protein alongside an engineered fusion of the cytokine receptor binding domain of JAK2 (Janus Kinase 2) to the catalytic domain from the CTK of interest, BLK. Upon activation of the cytokine receptor, the CRBD-CTK fusion phosphorylates the receptor, leading to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
Cytosolic Tyrosine Kinase
Sub Family:
Protein Tyrosine
Accession Number:
Target Name:
Target Aliases:
Growth hormone receptor, MODY11
Target Species:
Cell Background:
Product Type:
eXpress Assay Kits
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Assay Measures:
Functional Activity
Detection Method:
Additional Information

User Manuals & Protocols

70-304 PathHunter eXpress Cytosolic Tyrosine Kinase Activity Assay REV2 User Manual

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93-0882E3x Datasheet

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