PathHunter® eXpress ADCYAP1R1 Total GPCR Internalization Assay

Background Information:

The PathHunter® ADCYAP1R1 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. PACAP) based internalization of ADCYAP1R1 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress PK-tagged ADCYAP1R1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the ADCYAP1R1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

VIP and PACAP

Sub Family:

Class B

Coupling:

Gq & Gs

Protein Name:

PAC1 (PACAP)

Protein Aliases:

PACAP-R-1|PACAP-R1|PACAP receptor 1

Accession Number:

AY366498; NM_001118

UniProt Number:

P41586

Gene Name:

ADCYAP1R1

Gene ID:

117

Gene Aliases:

PAC1|PACAPR|PAC1R

Target Species:

Human

Cell Background:

U2OS