| Biological Information | |
|---|---|
| Background Information: | The PathHunter® EDG5 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. S1P) based internalization of EDG5 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged EDG5. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting EDG5-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. |
| Target Class: | GPCR |
| Family: | Lysophospholipid (S1P) Receptors |
| Sub Family: | Class A |
| Coupling: | Gs |
| Accession Number: | NM_004230.3 |
| Target Name: | EDG5 |
| Target Aliases: | Sphingosine 1-phosphate receptor 2, S1PR2, AGR16, DFNB68, EDG-5, EDG5, Gpcr13, H218, LPB2, S1P2 |
| Target Species: | Human |
| Cell Background: | U2OS |
| Usage | |
| Product Type: | Stable Cell Lines |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| β-Arrestin isoform: | β-Arrestin-2 |
| Assay Measures: | Internalization |
| Detection Method: | Chemiluminescence |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® EDG5 Activated GPCR Internalization U2OS Cell Line
The PathHunter® EDG5 Activated GPCR Internalization U2OS Cell Line measures EDG5 (GPCR) internalization via early-endosome localization. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-259 PathHunter Activated GPCR Internalization Assay REV6 User Manual
View DocumentDatasheets
93-0735C3 Datasheet
View Document