PathHunter® DRD1 Total GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® DRD1 Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. dopamine) based internalization of DRD1 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses PK-tagged DRD1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the DRD1 receptor to the early endosome leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Dopamine

Sub Family:

Class A

Coupling:

Gs

Protein Name:

D1

Protein Aliases:

Dopamine D1 receptor

Accession Number:

NM_000794.3

UniProt Number:

P21728

Gene Name:

DRD1

Gene ID:

1812

Target Species:

Human

Cell Background:

U2OS