Biological Information

Background Information:

The PathHunter® PROKR2 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prokinectin, EG-VEGF) based activation of PROKR2 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged PROKR2 and EA-tagged β-Arrestin 2. Activation of PROKR2 stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Prokineticin

Sub Family:

Class A

Protein Name:

PK2

Protein Aliases:

G-protein coupled receptor 73-like 1|G-protein coupled receptor I5E

Accession Number:

NM_144773

UniProt Number:

Q8NFJ6

Gene Name:

PROKR2

Gene ID:

128674

Gene Aliases:

GPR73b|PKR2|GPRg2|dJ680N4.3|GPR73L1

Target Species:

Human

Cell Background:

CHO-K1

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

Assay Measures:

β-Arrestin Recruitment

Detection Method:

Chemiluminescence

Additional Information

Brand:

PathHunter®