PathHunter® CHO-K1 PPARα Protein Interaction Cell Line

The PathHunter® CHO-K1 PPARα Protein Interaction Cell Line measures activated PPARα nuclear hormone receptor interaction with SRCPs. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0457C2 Size: 2 vials
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Biological Information
Background Information:
The PathHunter® PPARα Protein Interaction cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting NHR activation and recruitment of cofactors. This cell line enables assessment of ligand (e.g. leukotriene B4) based NHR activation via detection of SRCP (steroid receptor co-activator peptide) recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged PPARα NHR and EA-tagged steroid receptor co-activator peptide (SRCP). Upon activation of PPARα, induced interaction with SRCP leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
Nuclear Protein
Family:
NHR
Sub Family:
Non-Steroid
Accession Number:
NM_005036
Target Name:
PPARα
Target Aliases:
Peroxisome Proliferator Activated Receptor alpha
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Protein Interaction
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-263 PathHunter NHR Assays REV6 User Manual

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Datasheets

93-0457C2 Datasheet

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