Biological Information

Background Information:

The PathHunter® P2RY4 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. UTP) based activation of P2RY4 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged P2RY4 and EA-tagged β-Arrestin 2. Activation of P2RY4 stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

P2Y

Sub Family:

Class A

Coupling:

Gq

Protein Name:

P2Y4

Protein Aliases:

P2P|Uridine nucleotide receptor|UNR

Accession Number:

NM_002565

UniProt Number:

P51582

Gene Name:

P2RY4

Gene ID:

5030

Gene Aliases:

NRU|P2Y4|UNR|P2P

Target Species:

Human

Cell Background:

CHO-K1

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

β-Arrestin Isoform:

β-Arrestin-2

Assay Measures:

β-Arrestin Recruitment

Detection Method:

Chemiluminescence

Additional Information

Brand:

PathHunter®