Biological Information

Background Information:

The PathHunter® NPFFR1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. neuropeptide FF) based activation of NPFFR1 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged NPFFR1 and EA-tagged β-Arrestin 2. Activation of NPFFR1 stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Neuropeptide FF / Neuropeptide AF

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

NPFF1

Protein Aliases:

RFamide-related peptide receptor OT7T022

Accession Number:

NM_022146

UniProt Number:

Q9GZQ6

Gene Name:

NPFFR1

Gene ID:

64106

Gene Aliases:

GPR147|NPFF1

Target Species:

Human

Cell Background:

CHO-K1

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

β-Arrestin Isoform:

β-Arrestin-2

Assay Measures:

β-Arrestin Recruitment

Detection Method:

Chemiluminescence

Additional Information

Brand:

PathHunter®