Biological Information
Background Information:
The PathHunter® MRGPRX1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. BAM) based activation of MRGPRX1 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged MRGPRX1 and EA-tagged β-Arrestin 2. Activation of MRGPRX1 stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Class A Orphan
Sub Family:
Orphan and other 7TM
Coupling:
Gq
Protein Name:
MRGX1 (MRGPRX1)
Accession Number:
NM_147199
UniProt Number:
Q96LB2
Gene Name:
MRGPRX1
Gene ID:
259249
Gene Aliases:
MRGX1|SNSR3|SNSR4
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
β-Arrestin Isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®