PathHunter® CHO-K1 mADORA2B β-Arrestin Cell Line

The PathHunter® CHO-K1 mADORA2B β-Arrestin Cell Line measures mADORA2B (GPCR) activity via recruitment of β-Arrestin2. This cell line ships as two vials of cryopreserved cells. Mouse protein.

Catalog #: 93-0469C2 Size: 2 vials
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Biological Information
Background Information:
The PathHunter® mADORA2B cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. NECA) based activation of mADORA2B GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged mADORA2B and EA-tagged β-Arrestin 2. Activation of mADORA2B stimulates the recruitment of β-Arrestin 2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein.
Target Class:
GPCR
Family:
VIP and PACAP Receptors
Coupling:
Gs
Accession Number:
NM_007413
Target Name:
ADORA2B (A2B receptor)
Target Aliases:
Adenosine receptor A2B, A2BAR, A2BR, A2b, AA2BR, ARA2B
Target Species:
Mouse
Cell Background:
CHO-K1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
β-Arrestin isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-247 PathHunter Beta-Arrestin Assay for GPCR Cell Lines REV5 User Manual

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Datasheets

93-0469C2 Datasheet

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