Biological Information

Background Information:

The PathHunter® GPR109B cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. OEA) based activation of GPR109B GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged GPR109B and EA-tagged β-Arrestin 2. Activation of GPR109B stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Hydroxycarboxylic Acid

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

GPR109B

Protein Aliases:

G-protein coupled receptor HM74|G-protein coupled receptor HM74B|Niacin receptor 2|Nicotinic acid receptor 2|HCA3

Accession Number:

NM_006018

UniProt Number:

P49019

Gene Name:

HCAR3

Gene ID:

8843

Gene Aliases:

GPR109B|HCA3|HM74B|NIACR2

Target Species:

Human

Cell Background:

CHO-K1

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

β-Arrestin Isoform:

β-Arrestin-2

Assay Measures:

β-Arrestin Recruitment

Detection Method:

Chemiluminescence

Additional Information

Brand:

PathHunter®