PathHunter® CHO-K1 CXCR5 Bioassay Kit

Background Information:

The PathHunter® CHO-K1 CXCR5 Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. CXCL13) based activation of CXCR5 GPCR activity via detection of β-arrestin recruitment. The included cells overexpress ProLink-tagged CXCR5 and EA-tagged β-Arrestin-2. Activation of CXCR5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Protein Name:

CXCR5

Protein Aliases:

CXCR-5|Burkitt lymphoma receptor 1|Monocyte-derived receptor 15|MDR-15|CD antigen CD185

Accession Number:

NM_001716

UniProt Number:

P32302

Gene Name:

CXCR5

Gene ID:

643

Gene Aliases:

MDR15|CD185

Target Species:

Human

Cell Background:

CHO-K1