PathHunter® CHO-K1 CX3CR1 β-Arrestin Cell Line

The PathHunter® CHO-K1 CX3CR1 β-Arrestin Cell Line measures CX3CR1 (GPCR) activity via recruitment of β-Arrestin2. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0956C2 Size: 2 vials
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Biological Information
Background Information:
The PathHunter® CX3CR1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CX3CL1/fractalkine) based activation of CX3CR1 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged CX3CR1 and EA-tagged β-Arrestin 2. Activation of CX3CR1 stimulates the recruitment of β-Arrestin 2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Chemokine Receptors
Sub Family:
Class A
Coupling:
Gi/Go
Accession Number:
NM_001171174
Target Name:
CX3CR1
Target Aliases:
Chemokine C-X3-C receptor 1, CX3CR1, CX3CR1, Chemokine
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
β-Arrestin isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-247 PathHunter Beta-Arrestin Assay for GPCR Cell Lines REV5 User Manual

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Datasheets

93-0956C2 Datasheet

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PathHunter® Detection Kit
93-0001
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