PathHunter® CHO-K1 C5AR1 Bioassay Kit

Awaiting product image

The PathHunter® CHO-K1 C5AR1 Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. C5a) based activation of C5AR1 GPCR activity via detection of β-arrestin recruitment

Catalog #: 93-0557Y2-00059

$1,989.00 – $8,979.00

Inquire
Biological Information
Background Information:
The PathHunter® CHO-K1 C5AR1 Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. C5a) based activation of C5AR1 GPCR activity via detection of β-arrestin recruitment. The included cells overexpress ProLink-tagged C5AR1 and EA-tagged β-Arrestin-2. Activation of C5AR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.
Target Class:
GPCR
Family:
Complement Peptide Receptor (Anaphylotoxin)
Sub Family:
Class A
Accession Number:
NM_001736
Target Name:
C5AR1 (C5a1 receptor)
Target Aliases:
Complement component 5a receptor 1, C5A, C5AR, C5R1, CD88
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
Bioassay Kits
Application:
Potency; Commercial Release & Stability
Kit Components:
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Degradation
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-290 PathHunter GPCR Bioassay Kit REV11 User Manual

View Document

Datasheets

93-0557Y2x Datasheet

View Document