PathHunter® CHO-K1 BDKRB2 Bioassay Kit

Background Information:

The PathHunter® CHO-K1 BDKRB2 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. bradykinin) based activation of BDKRB2 GPCR via detection of β-arrestin recruitment. The included cells overexpress ProLink-tagged BDKRB2 and EA-tagged β-Arrestin-2. Activation of BDKRB2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Bradykinin

Sub Family:

Class A

Protein Name:

B2

Protein Aliases:

BK-2 receptor

Accession Number:

NM_000623.3

UniProt Number:

P30411

Gene Name:

BDKRB2

Gene ID:

624

Gene Aliases:

BK-2|BKR2

Target Species:

Human

Cell Background:

CHO-K1