PathHunter® CHO-K1 AGTR1 Bioassay Kit

Background Information:

The PathHunter® CHO-K1 AGTR1 Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. Angiotensin-II) based activation of AGTR1 GPCR activity via detection of β-arrestin recruitment. The included cells overexpress ProLink-tagged AGTR1 and EA-tagged β-Arrestin-2. Activation of AGTR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Angiotensin

Sub Family:

Class A

Protein Name:

AT1

Protein Aliases:

AT1BR|Angiotensin II type-1 receptor|AT1

Accession Number:

NM_000685

UniProt Number:

P30556

Gene Name:

AGTR1

Gene ID:

185

Gene Aliases:

AT1|AT2R1|AGTR1A|AT2R1A|HAT1R|AG2S|AT2R1B|AT1B

Target Species:

Human

Cell Background:

CHO-K1