Biological Information

Background Information:

The PathHunter® ADRA2B cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. epinephrine and norepinephrine) based activation of ADRA2B GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged ADRA2B and EA-tagged β-Arrestin 2. Activation of ADRA2B stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Adrenoceptor

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

alpha2B

Protein Aliases:

Alpha-2B adrenoreceptor|Alpha-2B adrenoceptor|Alpha-2BAR

Accession Number:

NM_000682.5; AY136748

UniProt Number:

P18089

Gene Name:

ADRA2B

Gene ID:

151

Gene Aliases:

ADRA2L1|ADRA2RL1

Target Species:

Human

Cell Background:

CHO-K1

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

β-Arrestin Isoform:

β-Arrestin-2

Assay Measures:

β-Arrestin Recruitment

Detection Method:

Chemiluminescence

Additional Information

Brand:

PathHunter®