PathHunter® CCR7 Activated GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® CCR7 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. CCL19/MIP-3β) based internalization of CCR7 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged CCR7. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting CCR7-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

CCR7

Protein Aliases:

CC-CKR-7|CCR-7|BLR2|CDw197|Epstein-Barr virus-induced G-protein coupled receptor 1|EBI1|EBV-induced G-protein coupled receptor 1|MIP-3 beta receptor|CD antigen CD197

Accession Number:

AY587876

UniProt Number:

P32248

Gene Name:

CCR7

Gene ID:

1236

Gene Aliases:

BLR2|CDw197|CD197

Target Species:

Human

Cell Background:

U2OS