PathHunter® AVPR2 Activated GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® AVPR2 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. vasopressin) based internalization of AVPR2 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged AVPR2. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting AVPR2-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Vasopressin and Oxytocin

Sub Family:

Class A

Coupling:

Gs

Protein Name:

V2

Protein Aliases:

nephrogenic diabetes insipidus

Accession Number:

NM_000054.4; NM_001146151

UniProt Number:

P30518

Gene Name:

AVPR2

Gene ID:

554

Gene Aliases:

ADHR|DIR|DIR3|V2R

Target Species:

Human

Cell Background:

U2OS