| Biological Information | |
|---|---|
| Background Information: | The PathHunter® Anakinra Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses IL-1α and IL-1β-induced dimerization of IL1RAP and IL1R1 receptors. This product uses EFC technology. The cell line included overexpresses IL1RAP target protein fused to PK alongside IL1R1 target protein fused to EA. Upon ligand-induced dimerization of IL1RAP and IL1R1, detectable EFC signal is produced. This assay has been optimized and qualified with Kineret® (not supplied in the bioassay kit). Kineret® is a registered trademark of Swedish Orphan Biovitrum AB. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay. |
| Target Class: | Cytokine |
| Family: | Immunoglobulin-like family of IL-1 Receptors |
| Accession Number: | NM_000877.3 |
| Target Name: | IL1R1/IL1RAP |
| Target Aliases: | IL-1A- IL1, IL-1A, IL1F1, IL1-ALPHA, IL-1 alpha, IL-1B- IL-1, IL1F2, IL1beta, IL1-BETA, Interleukin 1 receptor, type I |
| Target Species: | Human |
| Cell Background: | U2OS |
| Usage | |
| Product Type: | Bioassay Kits |
| Application: | Potency; Commercial Release & Stability |
| Kit Components: |  |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | Dimerization |
| Detection Method: | Chemiluminescence |
| Bioassay Data | |
| Qualified With: | Kineret® |
| Trademark Statement: | Kineret® is a registered trademark of Swedish Orphan Biovitrum AB |
| Clinical Relevance | |
| Therapeutic Area: | Inflammation/Allergy |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® Anakinra Bioassay Kit
The PathHunter® Anakinra Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses IL-1α and IL-1β-induced dimerization of IL1RAP and IL1R1 receptors.
View bioassay qualification data.
User Manuals & Protocols
70-393 PathHunter Anakinra Bioassay Kit REV0 User Manual
View DocumentDatasheets
93-1032Y3x Datasheet
View Document