PathHunter® Anakinra Bioassay Kit

Background Information:

The PathHunter® Anakinra Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses IL-1α and IL-1β-induced dimerization of IL1RAP and IL1R1 receptors. This product uses EFC technology. The cell line included overexpresses IL1RAP target protein fused to PK alongside IL1R1 target protein fused to EA. Upon ligand-induced dimerization of IL1RAP and IL1R1, detectable EFC signal is produced. This assay has been optimized and qualified with Kineret® (not supplied in the bioassay kit). Kineret® is a registered trademark of Swedish Orphan Biovitrum AB. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Please reach out to Tech Support at DiscoverXSupportUS@discovery.eurofinsus.com for lot specific CoA.

Target Class:

Cytokine Receptor

Family:

IL-1 Receptor

Protein Name:

IL1R1

Protein Aliases:

IL-1RT-1|IL-1RT1|CD121 antigen-like family member A|Interleukin-1 receptor alpha|IL-1R-alpha|Interleukin-1 receptor type I|p80|CD antigen CD121a [Cleaved into: Interleukin-1 receptor type 1, membrane form|mIL-1R1|mIL-1RI; Interleukin-1 receptor type 1, soluble form|sIL-1R1|sIL-1RI]

Accession Number:

NM_000877.3

UniProt Number:

P14778

Gene Name:

IL1R1

Gene ID:

3554

Gene Aliases:

IL1R|IL1RA|IL1RT1

Target Species:

Human

Cell Background:

U2OS