| Biological Information | |
|---|---|
| Background Information: | The PathHunter® Aflibercept Bioassay kit provides an easy-to-use cell based assay to determine Aflibercept potency and detect neutralizing antibodies. This bioassay assesses VEGFA-induced dimerization of VEGFR2 receptors. This product uses EFC technology. The included cells overexpress VEGFR2 target protein fused to PK alongside VEGFR2 target protein fused to EA. Upon ligand-induced dimerization of VEGFR2, detectable EFC signal is produced. This bioassay has been optimized and qualified with the marketed biologic drug, Eylea® which is not provided in the bioassay kit. Eyelea® is a registered trademark of Regeneron Pharmaceutical, Inc. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates |
| Target Class: | Kinase |
| Family: | Receptor Typosine Kinase (RTK): Growth Factor Receptor |
| Accession Number: | NM_002253.2 |
| Target Name: | KDR/KDR |
| Target Aliases: | Kinase Insert Domain Receptor, FLK1, CD309, VEGFR, VEGFR2 |
| Target Species: | Human |
| Cell Background: | HEK293 |
| Usage | |
| Product Type: | Bioassay Kits |
| Application: | Potency; Commercial Release & Stability |
| Kit Components: |  |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | Dimerization |
| Detection Method: | Chemiluminescence |
| Bioassay Data | |
| Qualified With: | Eylea® |
| Trademark Statement: | Eylea® is a registered trademark of Regeneron Pharmaceuticals, Inc. |
| Clinical Relevance | |
| Therapeutic Area: | Oncology/Immuno-Oncology |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® Aflibercept Bioassay Kit
The PathHunter® Aflibercept Bioassay kit provides an easy-to-use cell based assay to determine Aflibercept potency and detect neutralizing antibodies. This bioassay assesses VEGFA-induced dimerization of VEGFR2 receptors.
View bioassay qualification data.
User Manuals & Protocols
70-326 PathHunter Aflibercept Bioassay Kit User Manual REV5
View DocumentDatasheets
93-0996Y1x Datasheet
View Document