PathHunter® Adalimumab Bioassay Kit

The PathHunter® Adalimumab Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay is designed to measure IkB degradation as a result of TNF alpha-mediated activation of the NF-kB signaling pathway.

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Catalog #: 93-0538B15-00131

$1,989.00$9,979.00


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Biological Information
Background Information:
The PathHunter® Adalimumab Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay is designed to measure IkB degradation as a result of TNF alpha-mediated activation of the NF-kB signaling pathway. The included cells overexpress IκB tagged with PL. Addition of the detection reagent containing EA, forces complementation of the two enzyme fragments. The resulting active enzyme hydrolyzes the substrate to generate a chemiluminescent signal which is proportional to the degree of IκB stabilization. This assay has been optimized and qualified with Humira (not supplied in the bioassay kit). Humira is a registered trademark of AbbVie, Inc. The bioassay kit contains all the materials needed to run the assay, including cryopreserved cells, cell plating reagent, control agonist, detection reagents, and assay plates.
Target Class:
Cytokine
Family:
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Accession Number:
NM_020529
Target Name:
TNFa
Target Aliases:
IKBA, MAD-3, NFKBI, EDAID2, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Target Species:
Human
Cell Background:
A549
Usage
Product Type:
Bioassay Kits
Application:
Potency; Commercial Release & Stability
Kit Components:
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
SH2 Recruitment
Detection Method:
Chemiluminescence
Bioassay Data
Qualified With:
Humira®
Trademark Statement:
Humira® is a registered trademark of AbbVie, Inc.
Clinical Relevance
Therapeutic Area:
Inflammation/Allergy
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-412 PathHunter Adalimumab Bioassay Kit User Manual REV0

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Datasheets

93-0538B15x Datasheet

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