PathHunter® A549 IkappaB Degradation Cell Line

The PathHunter® A549 IκB Degradation Cell Line measures pathway activation-induced degradation of IκB target protein. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0538C15 Size: 2 vials


Biological Information
Background Information:
The PathHunter® A549 IκB Degradation Cell Line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting the NFκB pathway. This cell line enables detection of IκB levels, allowing for assessment of NFκB pathway activation-induced IκB degradation. This product uses EFC technology. The cell line included overexpresses IκB target protein fused to PL. EA is contained in the detection reagent. Upon pathway-activation-induced degradation of IκB EFC signal is lost. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
Signaling Pathway
Accession Number:
Target Name:
Target Aliases:
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitoralpha
Target Species:
Cell Background:
Product Type:
Stable Cell Lines
Drug Discovery & Development
Storage Conditions:
Short term (24 h): Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Assay Measures:
Detection Method:
Additional Information

User Manuals & Protocols

70-265 PathHunter Pathway Assay REV6 User Manual

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93-0538C15 Datasheet

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