| Biological Information | |
|---|---|
| Background Information: | The KILR® Retroparticles for Adherent & Suspension Cells (Hygromycin B) are MMLV particles designed to transduce adherent and suspension cells respectively. This allows production of PL-tagged, hygromycin B-resistant target cells of interest for use in cytotoxicity assays involving detection of cell lysis. This product uses EFC technology (https://www.discoverx.com/technologies-platforms/enzyme-fragment-complementation-technology). The retroparticle mix can be used to transduce target cells with a PL-tagged housekeeping gene. Target cell death in a cytotoxicity assay will release PL to the media, where EA contained in the detection reagent will produce EFC signal. These retroparticles ship as 2 vials each of cryopreserved adherent and suspension retroparticles. |
| Usage | |
| Product Type: | Retroparticles |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Short term (24 h): Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Measures: | Cytotoxicity |
| Detection Method: | Chemiluminescence |
| Clinical Relevance | |
| Therapeutic Area: | Oncology/Immuno-Oncology |
| Additional Information | |
| Brand: | KILR® |
KILR® Retroparticles for Adherent & Suspension Cells (Hygromycin B)
The KILR® Retroparticles for Adherent & Suspension Cells (Hygromycin B) are retroparticles for transducing cells of interest with PL to allow for cytotoxicity assays. This product contains 2 vials each of adherent / suspension retroparticles. Resists Hygromycin B.
User Manuals & Protocols
70-373 KILR Retroparticles For Cytotoxicity Assays REV2 User Manual
View DocumentDatasheets
97-0008 Datasheet
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