InCELL Hunter™ U2OS Bcl2-Bax Protein Binding Cell Line

The InCELL Hunter™ U2OS Bcl2-Bax Protein Binding Cell Line measures protein binding via compound-induced binding partner disruption and degradation. This cell line ships as two vials of cryopreserved cells.

Catalog #: 96-0010C3 Size: 2 vials

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Biological Information
Background Information:	The InCELL Hunter™ U2OS Bcl2-Bax Protein Binding Cell Line provides an easy-to-use cell based assay to study small molecule drug candidates that disrupt protein binding. This kit assesses compound-based disruption of Bcl2-Bax binding via detection of Bax levels. This product uses EFC technology. The cell line included overexpresses Bcl2 target in an untagged form and Bax binding partner fused to PL. When a compound engages the Bcl2 target protein, partner binding is disrupted and the destabilized Bax is degraded, leading to a loss of EFC signal upon addition of EA containing detection reagent. This stable modified cell line is provided as two vials of cryopreserved cells.
Family:	BCL2
Accession Number:	NM_000633
Target Name:	Bcl2-Bax
Target Aliases:	BCL2 B-cell CLL/lymphoma 2, BCL2 apoptosis regulator
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Target Engagement
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Target Engagement
Detection Method:	Chemiluminescence

Additional Information
Brand:	InCELL Hunter™