InCELL Hunter™ PKC-theta Kinase Cell Line (Jurkat)

The InCELL Hunter™ PKC-theta Kinase Cell Line (Jurkat) measures target engagement via compound-induced stabilization of PKC-theta target protein. This cell line ships as two vials of cryopreserved cells.

Catalog #: 96-0082C19 Size: 2 vials
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Biological Information
Background Information:
InCELL Hunter™ technology measures compound binding to an intracellular target by detecting changes in protein stability. In this assay, the target is fused to the enhanced ProLabel® (ePL) enzyme fragment and expressed in the selected cell background. The amount of fusion is quantified through the addition of substrate and complementary enzyme acceptor fragment (EA). The small size, but robust activity of the ePL tag makes it an ideal fusion partner. The small disordered peptide has minimal effects on target half-life, and generates high levels of enzyme activity, resulting in a sensitive measurement of protein levels.
Target Class:
Kinase
Family:
PKC
Sub Family:
Protein Ser/Thr
Accession Number:
NM_006257.3
Target Name:
Protein Kinase C theta
Target Aliases:
PKC-theta, PRKCT, nPKC-theta, PRKCQ
Target Species:
Human
Cell Background:
Jurkat
Usage
Product Type:
Stable Cell Lines
Application:
Target Engagement
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Target Engagement
Additional Information
Brand:
InCELL Hunter™

User Manuals & Protocols

70-400 InCELL Hunter Target Engagement Cell Lines REV0 User Manual

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Datasheets

96-0082C19 Datasheet

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