InCELL Hunter™ Human PKCθ Kinase Target Engagement Cell-Based Assay Kit (Jurkat)

Background Information:

The InCELL Hunter™ Human PKCθ Kinase Target Engagement Cell-Based Assay Kit (Jurkat) utilizes the InCELL Hunter™ PKCθ Kinase Binding cell line. The stable engineered cell line is intended for use in studying small molecule drug candidates that engage with kinases. This cell line enables assessment of compound-engagement-based stabilization of PKCθ via detection of PKCθ levels. This product uses EFC technology. The cell line included overexpresses PKCθ's kinase domain fused to PL. In the absence of compound that binds and stabilizes the target, this protein fragment is naturally degraded and no EFC signal is produced. When the compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Kinase

Family:

PKC

Sub Family:

Protein Ser/Thr

Protein Name:

PKCtheta

Protein Aliases:

nPKC-theta

Accession Number:

NM_006257.3

UniProt Number:

Q04759

Gene Name:

PRKCQ

Gene ID:

5588

Gene Aliases:

PRKCT

Target Species:

Human

Cell Background:

Jurkat