Biological Information

Background Information:

InCELL Hunter™ technology measures compound binding to an intracellular target by detecting changes in protein stability. In this assay, the target is fused to the enhanced ProLabel® (ePL) enzyme fragment and expressed in the selected cell background. The amount of fusion is quantified through the addition of substrate and complementary enzyme acceptor fragment (EA). The small size, but robust activity of the ePL tag makes it an ideal fusion partner. The small disordered peptide has minimal effects on target half-life, and generates high levels of enzyme activity, resulting in a sensitive measurement of protein levels.

Target Class:

Kinase

Family:

PKC

Sub Family:

Protein Ser/Thr

Protein Name:

PKCtheta

Protein Aliases:

nPKC-theta

Accession Number:

NM_006257.3

UniProt Number:

Q04759

Gene Name:

PRKCQ

Gene ID:

5588

Gene Aliases:

PRKCT

Target Species:

Human

Cell Background:

Jurkat

Usage

Product Type:

Stable Cell Lines

Application:

Target Engagement

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

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Assay Information

Assay Type:

Cell Based Binding

Assay Measures:

Target Engagement

Detection Method:

Chemiluminescence

Clinical Relevance

Therapeutic Area:

Oncology/Immuno-Oncology

Additional Information

Brand:

InCELL Hunter™