InCELL Hunter™ HEK 293 RIPK2 Kinase Binding Cell Line

Background Information:

The InCELL Hunter™ HEK 293 RIPK2 Kinase Binding Cell Line is a stable, engineered cell line for use in studying small molecule drug candidates that engage with kinases. This cell line enables assessment of compound-engagement-based stabilization of RIPK2 via detection of RIPK2 levels. This product uses EFC technology. The cell line included overexpresses RIPK2 target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is denatured by the heat treatment and no EFC signal is produced. When compound engages and heat-stabilizes the target, EA contained in the detection reagent produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Kinase

Family:

TKL

Sub Family:

Protein Ser/Thr

Protein Name:

RIPK2

Protein Aliases:

CARD-containing interleukin-1 beta-converting enzyme-associated kinase|CARD-containing IL-1 beta ICE-kinase|RIP-like-interacting CLARP kinase|Receptor-interacting protein 2|RIP-2|Tyrosine-protein kinase RIPK2

Accession Number:

NC_000008.11

UniProt Number:

O43353

Gene Name:

RIPK2

Gene ID:

8767

Gene Aliases:

RICK|RIP2|CARDIAK|CARD3

Target Species:

Human

Cell Background:

HEK293