InCELL Hunter™ HEK 293 BLK Kinase Binding Cell Line

The InCELL Hunter™ HEK 293 BLK Kinase Binding Cell Line measures target engagement via compound-induced stabilization of BLK target protein. This cell line ships as two vials of cryopreserved cells.

Catalog #: 96-0044C1 Size: 2 vials
Inquire
Biological Information
Background Information:
The InCELL Hunter™ HEK 293 BLK Kinase Binding Cell Line is a stable, engineered cell line for use in studying small molecule drug candidates that engage with kinases. This cell line enables assessment of compound-engagement-based stabilization of BLK via detection of BLK levels. This product uses EFC technology. The cell line included overexpresses BLK target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is denatured by the heat treatment and no EFC signal is produced. When compound engages and heat-stabilizes the target, EA contained in the detection reagent produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
Kinase
Family:
Cytosolic Tyrosine Kinase
Sub Family:
Protein Tyrosine
Accession Number:
NM_000163
Target Name:
Blk
Target Aliases:
Growth hormone receptor, MODY11
Target Species:
Human
Cell Background:
HEK293
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Target Engagement
Detection Method:
Chemiluminescence
Additional Information
Brand:
InCELL Hunter™

User Manuals & Protocols

70-400 InCELL Hunter Target Engagement Cell Lines REV0 User Manual

View Document

Datasheets

96-0044C1 Datasheet

View Document
AssayComplete™ Freezing Reagent F1
92-5101FR
2 x 50 mL

$339.00

AssayComplete™ Cell Culture Kit-105
92-3105G
Kit

$397.00

InCELL Detection Kit
96-0079
Inquire
AssayComplete™ Cell Detachment Reagent
92-0009
50 mL

$109.00

AssayComplete™ Thawing Reagent T1
92-4101TR
100 mL

$249.00