InCELL Hunter™ eXpress MEK1 Kinase Binding Assay

Background Information:

The InCELL Hunter™ eXpress MEK1 Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of MEK1 via detection of MEK1 levels. This product uses EFC technology. The cells included overexpress MEK1 target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

Kinase

Family:

STE

Sub Family:

Protein Ser/Thr

Protein Name:

MEK1

Protein Aliases:

MAPKK 1|MKK1|ERK activator kinase 1|MAPK/ERK kinase 1|MEK 1

Accession Number:

AAB97145.1

UniProt Number:

Q02750

Gene Name:

MAP2K1

Gene ID:

5604

Gene Aliases:

MEK1|MAPKK1|PRKMK1

Target Species:

Human

Cell Background:

A549