InCELL Hunter™ eXpress HEK 293 SMARCA4 Bromodomain Assay

Background Information:

The InCELL Hunter™ eXpress HEK 293 SMARCA4 Bromodomain Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of SMARCA4 via detection of SMARCA4 levels. This product uses EFC technology. The cells included overexpress SMARCA4 target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

Epigenetic

Family:

Bromodomain-Containing

Sub Family:

Reader

Protein Name:

SMARCA4

Protein Aliases:

SNF2-like 4|global transcription activator homologous sequence|sucrose nonfermenting-like 4|mitotic growth and transcription activator|BRM/SWI2-related gene 1|homeotic gene regulator|nuclear protein GRB1|brahma protein-like 1|ATP-dependent helicase SMARCA4

Accession Number:

NM_001128844

UniProt Number:

P51532

Gene Name:

SMARCA4

Gene ID:

6597

Gene Aliases:

hSNF2b|BRG1|BAF190|SNF2|SWI2|SNF2-BETA|SNF2LB|FLJ39786|BAF190A|SNF2B|SNF2L4

Target Species:

Human

Cell Background:

HEK293