InCELL Hunter™ eXpress HEK 293 BRD4(1) Bromodomain Assay

Background Information:

The InCELL Hunter™ eXpress HEK 293 BRD4(1) Bromodomain Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of BRD4(1) via detection of BRD4(1) levels. This product uses EFC technology. The cells included overexpress BRD4(1) target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

Epigenetic

Family:

Bromodomain-Containing

Sub Family:

Reader

Protein Name:

BRD4

Protein Aliases:

chromosome-associated protein

Accession Number:

NM_058243

UniProt Number:

O60885

Gene Name:

BRD4

Gene ID:

23476

Gene Aliases:

HUNKI|MCAP|CAP|HUNK1

Target Species:

Human

Cell Background:

HEK293