InCELL Hunter™ eXpress HCK Kinase Binding Assay

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The InCELL Hunter™ eXpress HCK Kinase Binding Assay measures target engagement via compound-induced stabilization of HCK target protein. eXpress kits contain all assay materials: cells, reagents, and plates.

Catalog #: 96-0041E15CP0L

$1,789.00 – $5,989.00

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Biological Information
Background Information:
The InCELL Hunter™ eXpress HCK Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of HCK via detection of HCK levels. This product uses EFC technology. The cells included overexpress HCK target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
Kinase
Family:
Cytosolic Tyrosine Kinase
Sub Family:
Protein Tyrosine
Accession Number:
NC_000020.11
Target Name:
Hck
Target Aliases:
JTK9, p59Hck, p61Hck
Target Species:
Human
Cell Background:
A549
Usage
Product Type:
eXpress Assay Kits
Application:
Target Engagement
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Target Engagement
Detection Method:
Chemiluminescence
Additional Information
Brand:
InCELL Hunter™

User Manuals & Protocols

70-429 InCELL Hunter eXpress Assays User Manual REV0A

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Datasheets

96-0041E15x Datasheet

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