InCELL Hunter™ eXpress HCK Kinase Binding Assay

Background Information:

The InCELL Hunter™ eXpress HCK Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of HCK via detection of HCK levels. This product uses EFC technology. The cells included overexpress HCK target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

Kinase

Family:

TK

Sub Family:

Protein Tyrosine

Protein Name:

HCK

Protein Aliases:

Hematopoietic cell kinase|Hemopoietic cell kinase|p59-HCK/p60-HCK|p59Hck|p61Hck

Accession Number:

NC_000020.11

UniProt Number:

P08631

Gene Name:

HCK

Gene ID:

3055

Gene Aliases:

JTK9

Target Species:

Human

Cell Background:

A549