InCELL Hunter™ eXpress BLK Kinase Binding Assay

The InCELL Hunter™ eXpress BLK Kinase Binding Assay measures target engagement via compound-induced stabilization of BLK target protein. eXpress kits contain all assay materials: cells, reagents, and plates.

Catalog #: 96-0044E1CP0L


Biological Information
Background Information:
The InCELL Hunter™ eXpress BLK Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of BLK via detection of BLK levels. This product uses EFC technology. The cells included overexpress BLK target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
Cytosolic Tyrosine Kinase
Sub Family:
Protein Tyrosine
Accession Number:
Target Name:
Target Aliases:
Growth hormone receptor, MODY11
Target Species:
Cell Background:
Product Type:
eXpress Assay Kits
Target Engagement
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Assay Measures:
Target Engagement
Detection Method:
Additional Information
InCELL Hunter™

User Manuals & Protocols

70-429 InCELL Hunter eXpress Assays User Manual REV0A

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96-0044E1x Datasheet

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