InCELL Hunter™ eXpress Bcl2L1-Bax Protein Binding Assay

Background Information:

The InCELL Hunter™ eXpress Bcl2L1-Bax Protein Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that disrupt protein binding. This kit assesses compound-based disruption of Bcl2L1-Bax binding via detection of Bax levels. This product uses EFC technology. The cells included overexpress Bcl2L1 target in an untagged form and Bax binding partner fused to PL. When a compound engages the Bcl2L1 target protein, partner binding is disrupted and the destabilized Bax is degraded, leading to a loss of EFC signal upon addition of EA containing detection reagent. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

Cytosolic (Other)

Family:

Bcl-2

Sub Family:

Apoptosis

Protein Name:

B2CL1

Protein Aliases:

Apoptosis regulator Bcl-X

Accession Number:

NM_138578.1

UniProt Number:

Q07817

Gene Name:

BCL2L1

Gene ID:

598

Gene Aliases:

BCL2L|BCLX

Target Species:

Human

Cell Background:

U2OS