InCELL Hunter™ A549 HCK Kinase Binding Cell Line

The InCELL Hunter™ A549 HCK Kinase Binding Cell Line measures target engagement via compound-induced stabilization of HCK target protein. This cell line ships as two vials of cryopreserved cells.

Catalog #: 96-0041C15 Size: 2 vials

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Biological Information
Background Information:	The InCELL Hunter™ A549 HCK Kinase Binding Cell Line is a stable, engineered cell line for use in studying small molecule drug candidates that engage with kinases. This cell line enables assessment of compound-engagement-based stabilization of HCK via detection of HCK levels. This product uses EFC technology. The cell line included overexpresses HCK target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is denatured by the heat treatment and no EFC signal is produced. When compound engages and heat-stabilizes the target, EA contained in the detection reagent produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	Kinase
Family:	Cytosolic Tyrosine Kinase
Sub Family:	Protein Tyrosine
Accession Number:	NC_000020.11
Target Name:	Hck
Target Aliases:	JTK9, p59Hck, p61Hck
Target Species:	Human
Cell Background:	A549

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Target Engagement
Detection Method:	Chemiluminescence

Additional Information
Brand:	InCELL Hunter™