Biological Information

Background Information:

The InCELL Hunter™ A549 GLP Methyltransferase Cell Line is a stable, engineered cell line for use in studying small molecule drug candidates that engage with regulators of epigenetics pathways. This cell line enables assessment of compound-engagement-based stabilization of GLP via detection of GLP levels. This product uses EFC technology. The cell line included overexpresses GLP target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Epigenetic

Family:

Histone Methyltransferase (HMT)

Sub Family:

Writer

Protein Name:

EHMT1

Protein Aliases:

GLP

Accession Number:

NM_024757

UniProt Number:

Q9H9B1

Gene Name:

EHMT1

Gene ID:

79813

Gene Aliases:

Eu-HMTase1|FLJ12879|KIAA1876|bA188C12.1|KMT1D

Target Species:

Human

Cell Background:

A549

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based Binding

Assay Measures:

Target Engagement

Detection Method:

Chemiluminescence

Clinical Relevance

Therapeutic Area:

Metabolic Diseases

Additional Information

Brand:

InCELL Hunter™