Biological Information | |
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Background Information: | The InCELL Hunter™ A549 G9a Methyltransferase Cell Line is a stable, engineered cell line for use in studying small molecule drug candidates that engage with regulators of epigenetics pathways. This cell line enables assessment of compound-engagement-based stabilization of G9a via detection of G9a levels. This product uses EFC technology. The cell line included overexpresses G9a target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | Epigenetic Protein |
Family: | Histone methyltransferase |
Accession Number: | NM_006709 |
Target Name: | G9a |
Target Aliases: | Euchromatic histone-lysine N-methyltransferase 2 |
Target Species: | Human |
Cell Background: | A549 |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | Target Engagement |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | InCELL Hunter™ |
InCELL Hunter™ A549 G9a Methyltransferase Cell Line
The InCELL Hunter™ A549 G9a Methyltransferase Cell Line measures target engagement via compound-induced stabilization of G9a target protein. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-400 InCELL Hunter Target Engagement Cell Lines REV0 User Manual
View DocumentDatasheets
96-0003C15 Datasheet
View Document