InCELL Hunter™ A549 CLK2 Kinase Binding Cell Line

The InCELL Hunter™ A549 CLK2 Kinase Binding Cell Line measures target engagement via compound-induced stabilization of CLK2 target protein. This cell line ships as two vials of cryopreserved cells.

Catalog #: 96-0057C15 Size: 2 vials
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Biological Information
Background Information:
The InCELL Hunter™ A549 CLK2 Kinase Binding Cell Line is a stable, engineered cell line for use in studying small molecule drug candidates that engage with kinases. This cell line enables assessment of compound-engagement-based stabilization of CLK2 via detection of CLK2 levels. This product uses EFC technology. The cell line included overexpresses CLK2 target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is denatured by the heat treatment and no EFC signal is produced. When compound engages and heat-stabilizes the target, EA contained in the detection reagent produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
Kinase
Family:
CMGC Kinase
Sub Family:
Protein Ser/Thr
Accession Number:
NC_000001.11
Target Name:
CLK2
Cell Background:
A549
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Target Engagement
Detection Method:
Chemiluminescence
Additional Information
Brand:
InCELL Hunter™

User Manuals & Protocols

70-400 InCELL Hunter Target Engagement Cell Lines REV0 User Manual

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Datasheets

96-0057C15 Datasheet

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