CHO-K1 PTAFR Gq Cell Line

The CHO-K1 PTAFR Gq Cell Line measures PTAFR (GPCR) activation via an increase in intracellular calcium. This cell line ships as two vials of cryopreserved cells.

Catalog #: 95-0091C2 Size: 2 vials
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Biological Information
Background Information:
The PTAFR Gq cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. PAF) based activation of PTAFR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTAFR , a naturally Gq coupled GPCR. Upon activation of PTAFR , the resulting calcium activity can be fluorescently assayed using the Calcium NoWash PLUS Detection Kit. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Platelet-activating factor
Sub Family:
Class A
Coupling:
Gi/Go & Gq
Accession Number:
NM_000952.4
Target Name:
PTAFR
Target Aliases:
Platelet activating receptor, PAFR
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Calcium Flux
Detection Method:
Chemiluminescence
Additional Information
Brand:
DiscoverX

User Manuals & Protocols

70-296 Native Calcium GPCR Assays REV2 User Manual

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Datasheets

95-0091C2 Datasheet

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