ChemiSCREEN™ PAR2 Receptor Stable Cell Line

Background Information:

The ChemiSCREEN™ PAR2 Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. PAR2) based activation of F2RL1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses F2RL1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of F2RL1 , the resulting calcium activity can be fluorescently assayed.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Proteinase-Activated

Sub Family:

Class A

Protein Name:

PAR2

Protein Aliases:

proteinase-activated receptor-2

Accession Number:

NM_005242.4

UniProt Number:

P55085

Gene Name:

F2RL1

Gene ID:

2150

Gene Aliases:

PAR2|GPR11

Target Species:

Human

Cell Background:

Chem-1