ChemiSCREEN™ GABAB Receptor Stable Cell Line

Background Information:

The ChemiSCREEN™ GABAB Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. GABA) based activation of GABBR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GABBR1 alongside partner subunit GABBR2 and endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of GABBR1 , the resulting calcium activity can be fluorescently assayed.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

GABAB

Sub Family:

Class C

Protein Name:

GABAB (B1/B2)

Protein Aliases:

GABA-B receptor

Accession Number:

NM_021903; NM_005458

UniProt Number:

Q9UBS5

Gene Name:

GABBR1

Gene ID:

2550

Gene Aliases:

hGB1a|GPRC3A

Target Species:

Human

Cell Background:

Chem-1