ChemiSCREEN™ CRF2 Receptor Stable Cell Line

Background Information:

The ChemiSCREEN™ CRF2 Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CRH, sauvagine) based activation of CRHR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CRHR2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CRHR2 , the resulting calcium activity can be fluorescently assayed.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Corticotropin-Releasing Factor

Sub Family:

Class B

Protein Name:

CRF2

Protein Aliases:

CRF-R2|CRFR-2|Corticotropin-releasing hormone receptor 2|CRH-R-2|CRH-R2

Accession Number:

NM_001883

UniProt Number:

Q13324

Gene Name:

CRHR2

Gene ID:

1395

Gene Aliases:

CRF2|CRF-RB|HM-CRF|CRF2R|CRH2R

Target Species:

Human

Cell Background:

Chem-1