ChemiSCREEN™ alpha1D Adrenergic Receptor Stable Cell Line

Background Information:

The ChemiSCREEN™ α1D Adrenergic Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. epinephrine and norepinephrine) based activation of ADRA1D GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADRA1D (lacking residues 2-79) alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of ADRA1D , the resulting calcium activity can be fluorescently assayed.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Adrenoceptor

Sub Family:

Class A

Protein Name:

alpha1D

Protein Aliases:

Alpha-1D adrenoreceptor|Alpha-1D adrenoceptor|Alpha-adrenergic receptor 1a

Accession Number:

NM_000678

UniProt Number:

P25100

Gene Name:

ADRA1D

Gene ID:

146

Gene Aliases:

ADRA1R|ADRA1A|ADRA1

Target Species:

Human

Cell Background:

Chem-1