ChemiSCREEN™ 5-HT4B Serotonin Receptor Stable Cell Line

Background Information:

The ChemiSCREEN™ 5-HT4B Serotonin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin/5-HT) based activation of HTR4B GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses HTR4B alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of HTR4B , the resulting calcium activity can be fluorescently assayed.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Serotonin

Sub Family:

Class A

Protein Name:

5-HT4

Accession Number:

NM_000870

UniProt Number:

Q13639

Gene Name:

HTR4

Gene ID:

3360

Gene Aliases:

5-HT4

Target Species:

Human

Cell Background:

Chem-1