cAMP Hunter™ Teriparatide Bioassay Kit

Background Information:

The cAMP Hunter™ Teriparatide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Teriparatide based activation of PTHR GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTHR, a naturally Gs coupled GPCR. Upon activation of PTHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This assay has been optimized and qualified with ‎Forteo® (not supplied in the bioassay kit). ‎Forteo® is a registered trademark of Eli Lilly and Company. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Please reach out to Tech Support at DiscoverXSupportUS@discovery.eurofinsus.com for lot specific CoA.

Target Class:

GPCR

Family:

Parathyroid Hormone

Sub Family:

Class B

Protein Name:

PTH1

Protein Aliases:

PTH/PTHr receptor|Parathyroid hormone 1 receptor|PTH1 receptor

Accession Number:

AY449732

UniProt Number:

Q03431

Gene Name:

PTH1R

Gene ID:

5745

Target Species:

Human

Cell Background:

CHO-K1