| Biological Information | |
|---|---|
| Background Information: | The cAMP Hunter™ Semaglutide Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. Exendin-4) based on activation of GLP1R GPCR activity via detection of cAMP. The included cell line overexpresses ProLink-tagged GLP1R and EA-tagged . Activation of GLP1R stimulates the recruitment of and produces EFC signal. This assay has been optimized and qualified with Semaglutide (not supplied in the bioassay kit). Ozempic® is a registered trademark of Novo Nordisk A/S. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. |
| Target Class: | GPCR |
| Family: | Glucagon Receptor Family |
| Sub Family: | Class B |
| Accession Number: | NM_002062.3 |
| Target Name: | GLP1R |
| Target Aliases: | Glucagon-like peptide receptor 1GLP-1GLP-1-RGLP-1R |
| Target Species: | Human |
| Cell Background: | CHO-K1 |
| Usage | |
| Product Type: | Bioassay Kits |
| Application: | Potency, Commercial Release & Stability |
| Kit Components: |  |
| Storage Conditions: | Short term (24 h): Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | cAMP Detection |
| Detection Method: | Chemiluminescence |
| Bioassay Data | |
| Qualified With: | Ozempic® |
| Trademark Statement: | Ozempic® is a registered trademark of Novo Nordisk A/S |
| Clinical Relevance | |
| Therapeutic Area: | Metabolic Diseases |
| Additional Information | |
| Brand: | cAMP Hunter™ |
cAMP Hunter™ Semaglutide Bioassay Kit
The cAMP Hunter™ Semaglutide Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. Exendin-4) based on activation of GLP1R GPCR activity via detection of cAMP.
User Manuals & Protocols
70-441 cAMP Hunter Semaglutide Bioassay Kit User Manual Rev0
View DocumentDatasheets
95-0062Y2x Datasheet
View Document